Detection of multidrug-resistant (MDR) Staphylococcus aureus and coagulase-negative staphylococci (CoNS) in cow milk and hands of farmers in East Java, Indonesia

Abstract. Khairullah AR, Kurniawan SC, Sudjarwo SA, Effendi MH, Afnani DA, Silaen OSM, Putra GDS, Riwu KHP, Widodo A, Ramandinianto SC. 2023. Detection of multidrug-resistant Staphylococcus aureus and coagulase-negative staphylococci in cow milk hands farmers East Java, Indonesia. Biodiversitas 24: 658-664. Cow is a nutrient-dense food containing various nutrients, i.e., carbohydrates, fat, protein. However, it can potentially spread several pathogenic bacteria, such as staphylococci. Staphylococci cause public health problems. Staphylococcal strains were divided into two groups based on coagulase characteristics, namely coagulase-positive (CoNS). Coagulase-positive was represented by aureus. S. CoNS become resistant to antibiotics, known multidrug resistance (MDR) bacteria. bacteria are causal agents human infections. This study aims identify the presence dairy farms We collected 332 125 swab samples from hand Probolinggo, Tulungagung, Blitar The tested for antibiotic susceptibility using disc diffusion method. used cefoxitin, erythromycin, gentamicin, tetracycline, oxacillin. results isolation identification morphological biochemical characteristics showed that out 457 samples, 291 (63.68%) positive aureus, 166 (36.32%) CoNs. There 15 isolates (5.15%) 7 (4.22%) confirmed MDR; therefore, drugs very high province Java. Intensive livestock systems, densities, excessive use antibiotics modern systems may predispose developing growth MDR In conclusion, this indicate Java provinces. be prevented spreading improving biosecurity, hygiene milking process, regular care animals workers.